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KMID : 0613820170270050517
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Journal of Life Science
2017 Volume.27 No. 5 p.517 ~ p.523
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Effects of Arsenite and Antioxidants on Sperm Motility, Plasma Membrane Integrity, Mitochondrial Activity, and Lipid Peroxidation in Pigs
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Kim Han-Su
Lee You-Sub
Lee Sang-Hee
Cheong Hee-Tae
Park Choon-Keun
Lee Seung-Hyung
Yang Boo-Keun
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Abstract
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This study was to investigate the role of antioxidants on the characteristics of arsenite-damaged boar semen. Collected sperm was diluted with semen extender, and 100 μM arsenite was used for sperm damage. Then melatonin, silymarin, curcumin, and vitamin E were applied for 3, 6, and 9 hr in arsenite-treated boar sperm. Sperm characteristics were then analyzed for motility, plasma membrane integrity, mitochondrial activity, and lipid peroxidation. In the results, sperm motility (control, 77.3± 1.8%) was decreased by arsenite (33.3±1.5%), while the antioxidant treatment groups (100 nM melatonin, 55.8±3.4%; 2 μM silymarin, 48.8±3.4%; 10 μM curcumin, 53.9±2.8%; and 500 μM vitamin E, 54.5±3.1%) showed increases compared to the arsenite group (p<0.05). 100 μM arsenite decreased the sperm plasma membrane integrity (24.5±1.6%) and mitochondrial activity (58.2±2.6%), and increased lipid peroxidation (5.3±0.2%) at 3 hr (p<0.05). However, arsenite-treated samples with 100 nM melatonin, 2 μM silymarin, 10 μM curcumin, and 500 μM vitamin E increased the plasma membrane integrity and mitochondria activity, and decreased lipid peroxidation compared to the arsenite-treated samples. In summary, arsenite may induce sperm damage and oxidation stress, while antioxidants such as melatonin, silymarin, curcumin, and vitamin E are useful for maintaining sperm characteristics. Therefore, antioxidants can protect sperm against damage by arsenite in fresh boar semen.
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KEYWORD
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Arsenite, curcumin, melatonin, silymarin, sperm, vitamin E
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